Leucine regulates porcine muscle fiber type transformation via adiponectin signaling pathway

Leucine can promote slow-twitch muscle fibers formation, and this effect may be mediated by AMPK signaling pathway. In addition, adiponectin (AdipoQ) plays an important role in regulation of muscle fiber type transformation. AdipoQ is located in the upstream of AMPK and its secretion can be regulated by leucine. Therefore, the aim of this study was to explore whether leucine affects muscle fiber type transformation through AdipoQ signaling pathway. Our data showed that 4 mM leucine significantly increased protein expression levels of slow MyHC, Myoglobin, Troponin I-SS, AdipoQ, AdipoR1, phospho-AMPK (p-AMPK) and PGC-1 alpha and mRNA expression levels of AMPK alpha 2, PGC-1 alpha, AdipoQ and AdipoR1, and significantly decreased fast MyHC protein expression. In addition, 4 mM leucine significantly increased the SDH activity while significantly decreased the LDH activity. However, knockdown of AdipoR1 expression by AdipoR1-siRNA abolished leucine-induced upregulation of protein expressions of slow MyHC, AdipoR1, p-AMPK, PGC-1 alpha and NRF1, mRNA expressions of MyHC I, MyHC IIa, AdipoR1, AMPK alpha 2 and PGC-1 alpha, ATP5G, TFAM and NRF1, and mtDNA level, as well as downregulation of protein expression of fast MyHC and mRNA expression of MyHC IIb. Together, our data revealed that leucine promotes muscle fiber type transformation from fast-twitch to slow-twitch through AdipoQ signaling pathway.

Automated summary

The study reveals that leucine promotes muscle fiber type transformation from fast-twitch to slow-twitch through the AdipoQ signaling pathway. This is achieved by increasing the expression of slow muscle markers and decreasing the expression of fast muscle markers. Leucine plays a significant role in regulating muscle fiber type transformation.