Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 60, Issue 21, Pages 11884-11891Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202015948
Keywords
2D NMR spectroscopy; CEST; NOESY; RNA; SARS-CoV-2
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Funding
- EU Horizon 2020 program (FET-OPEN Grant) [828946]
- Israel Science Foundation [965/18, 3572/20]
- Perlman Family Foundation
- Goethe Corona Funds
- EU
- DFG [902]
- state of Hesse
- German-Israel Foundation [G-1501-302]
- Projekt DEAL
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The study introduces an alternative method, Selective Magnetization Transfer (SMT), based on encoding a priori selected peaks by selective saturations, to enhance NOEs between labile sites in RNA, particularly imino-imino cross-peaks. This method plays a crucial role in structural NMR and accelerates the NMR resolution of RNA structures.
2D NOESY plays a central role in structural NMR spectroscopy. We have recently discussed methods that rely on solvent-driven exchanges to enhance NOE correlations between exchangeable and non-exchangeable protons in nucleic acids. Such methods, however, fail when trying to establish connectivities within pools of labile protons. This study introduces an alternative that also enhances NOEs between such labile sites, based on encoding a priori selected peaks by selective saturations. The resulting selective magnetization transfer (SMT) experiment proves particularly useful for enhancing the imino-imino cross-peaks in RNAs, which is a first step in the NMR resolution of these structures. The origins of these enhancements are discussed, and their potential is demonstrated on RNA fragments derived from the genome of SARS-CoV-2, recorded with better sensitivity and an order of magnitude faster than conventional 2D counterparts.
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