Journal
ANALYTICAL CHEMISTRY
Volume 93, Issue 10, Pages 4666-4675Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c00239
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Funding
- Max Planck Society
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The study of synthetic oligo- and polysaccharides, which require pure compounds with defined structures obtained from chemical or enzymatic syntheses, can be effectively analyzed using wide mass range ultrahigh-resolution MALDI-FT-ICR mass spectrometry. Detection of fragment ions generated from glycosidic bond cleavage provides information on monosaccharide content and linkage type, enabling accurate analysis of carbohydrate compositions and structures.
Carbohydrates, such as oligo- and polysaccharides, are highly abundant biopolymers that are involved in numerous processes. The study of their structure and functions is commonly based on a material that is isolated from complex natural sources. However, a more precise analysis requires pure compounds with well-defined structures that can be obtained from chemical or enzymatic syntheses. Novel synthetic strategies have increased the accessibility of larger monodisperse polysaccharides, posing a challenge to the analytical methods used for their molecular characterization. Here, we present wide mass range ultrahigh-resolution matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) as a powerful platform for the analysis of synthetic oligo- and polysaccharides. Synthetic carbohydrates 16-, 64-, 100-, and 151-mers were mass analyzed and characterized by MALDI in-source decay FT-ICR MS. Detection of fragment ions generated from glycosidic bond cleavage (or cross-ring cleavage) provided information of the monosaccharide content and the linkage type, allowing for the corroboration of the carbohydrate compositions and structures.
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