Accurate In Situ Monitoring of Mitochondrial H2O2 by Robust SERS Nanoprobes with a Au-Se Interface

Mitochondrial redox homeostasis plays a vital role in many biological processes. Hydrogen peroxide (H2O2), one of the most important components for the balance between oxidizing species and reducing species, also acts as the messenger of mitochondrial damage. Thus, an accurate in situ quantitative detection of H2O2 in mitochondria is very important for the evaluation of mitochondrial redox homeostasis. Here, we develop robust surface-enhanced Raman spectroscopy (SERS) nanoprobes based on Au nanoparticles as SERS substrate and functionalized carrier, which is further modified with a phenylboronic acid pinacol ester for specific H2O2 response and a location peptides for mitochondrial targeting by creating a robust Au-Se interface. The SERS nanoprobes show good resistance to abundant thiol under biological conditions and superior performance for mitochondria H2O2 monitoring in living cells than the SERS nanoprobes with the traditional Au-S interface, which enables us to achieve in situ quantification of mitochondrial H2O2 and obtain its real-time dynamic change under oxidative stress.

Automated summary

In this study, robust surface-enhanced Raman spectroscopy (SERS) nanoprobes based on Au nanoparticles were developed for monitoring mitochondrial H2O2 in living cells, showing superior performance compared to traditional nanoprobes.