Journal
ANALYTICAL CHEMISTRY
Volume 93, Issue 5, Pages 2988-2995Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.0c05011
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [21974078, 21727813]
- National Key RD Program [2019YFA0508403]
- Natural Science Foundation of China [31871443]
- Tsinghua University Tutor Research Fund
Ask authors/readers for more resources
The study presents a novel method for real-time monitoring of phase-separated condensates with good penetration and spatiotemporal resolution. Using this approach, researchers successfully explored the dynamic process of phase separation in fused in sarcoma protein.
The formation of biomolecular condensates is driven by liquid-liquid phase separation, which is prevalent in cells to govern crucial cellular functions. However, understanding the properties of phase-separated condensates remains very challenging for the lack of suitable techniques. Here, we report a photoluminescence lifetime imaging method for real-time monitoring of phase-separated condensates, both in vitro and in living cells, using a microsecond-scale photoluminescence lifetime probe based on iridium complex. The probe has a large Stokes shift, excellent cell permeability, and minimal cell autofluorescence interference. With this method, the dynamic process of phase separation of fused in sarcoma protein has been well explored, showing high spatiotemporal resolution and high throughput. Beginning with initial formation, the protein droplets get bigger and more viscous, and then a final maturation to solidified aggregates has been characterized. This study paves the path for a deeper understanding of the properties of phase-separated biomolecular condensates.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available