Journal
ANALYTICAL CHEMISTRY
Volume 93, Issue 11, Pages 4909-4915Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.0c05187
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Funding
- National Natural Science Foundation of China [21427808]
- Fundamental Research Funds of Shandong University [ZY202006]
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The study demonstrated successful achievement of aqueous monochromatic ECL with Met-capped Au-Ag BNCs as luminophores and TEOA as coreactant, resulting in efficient NIR-II ECL and its application in immunoassays. A sandwich immunocomplex immobilization strategy allowed selective detection of CA125 with a detection limit of 5 x 10(-5) U/mL.
Aqueous electrochemiluminescence (ECL) in the second near-infrared biowindow (NIR-II, 900-1700 nm) was anticipated for ECL evolution and spectral multiplexing. Herein, aqueous and monochromatic ECL with a single emission peak beyond 900 nm was achieved by employing methionine (Met)-capped Au-Ag bimetallic nanoclusters (BNCs) as luminophores and triethanolamine (TEOA) as a coreactant. The Met-capped Au-Ag BNCs with surface-defect-induced PL around 756 nm were water-soluble and synthesized via doping Met-capped Au NCs with Ag in a doping-in-growth way. By extensively exploiting the red-shifting nature of surface-defect-induced ECL to PL and the synergetic-effect-enhanced ECL of BNCs, physically surface-confined Au-Ag BNCs exhibited efficient NIR-II ECL around 906 nm in aqueous medium. A spectrum-based NIR-II ECL immunoassay around 915 nm was also achieved by immobilizing the Au-Ag BNCs onto an electrode surface via forming a sandwich immunocomplex, which could selectively determine CA125 from 5 x 10(-4) to 1 U/mL with a detection limit of 5 x 10(-5) U/mL (S/N = 3). The combined strategy of surface-defect-induced ECL and synergetic-effect-enhanced ECL would enable promising biorelated application of NIR-II ECL.
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