Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 413, Issue 9, Pages 2503-2511Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-021-03205-x
Keywords
Cyantraniliprole; Chlorantraniliprole; Nanobody; Immunoassay; Bok choy; Soil
Funding
- Key Project of Inter-Governmental International Scientific and Technological Innovation Cooperation [2016YFE0108900, 2019YFE0115800]
- National Key Research and Development Program of China [2016YFD0800606, 2018YFC1602900]
- National Institute of Environmental Health Sciences (NIEHS) Superfund Research Program [P42ES04699]
- NIEHS RIVER Award [R35ES030443]
- USDA Hatch Project [HAW05044-R]
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In this study, camel-derived nanobodies recognizing cyantraniliprole and chlorantraniliprole were successfully generated, leading to the development of ELISA assays for detection of these insecticides. The ELISA results showed that chlorantraniliprole had higher concentrations than cyantraniliprole in soil and vegetables, indicating its longer persistence in the environment.
Cyantraniliprole and chlorantraniliprole are anthranilic diamide insecticides acting on ryanodine receptors. In this study, two camel-derived nanobodies (Nbs, named C1 and C2) recognizing cyantraniliprole as well as chlorantraniliprole were generated. C1-based enzyme-linked immunosorbent assays (ELISAs) for the detection of the two insecticides were developed. The half-maximum signal inhibition concentrations (IC50) of cyantraniliprole and chlorantraniliprole by ELISA were 1.2 and 1.5 ng mL(-1), respectively. This assay was employed to detect these two insecticides in soil and vegetables. The average recoveries of cyantraniliprole from both bok choy (Brassica chinensis L.) and soil samples were 90-129%, while those of chlorantraniliprole were in a range of 89-120%. The insecticide residues in soil and bok choy, which were collected from plots sprayed with cyantraniliprole and chlorantraniliprole, were simultaneously detected by the resulting ELISA and a high-performance liquid chromatography (HPLC) method, showing a satisfactory correlation. Higher concentrations of chlorantraniliprole than cyantraniliprole were detected in soil and vegetables, which indicates the longer persistence of chlorantraniliprole in the environment.
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