The transcriptome-wide landscape of molecular subtype-specific mRNA expression profiles in acute myeloid leukemia

Molecular classification of acute myeloid leukemia (AML) aids prognostic stratification and clinical management. Our aim in this study is to identify transcriptome-wide mRNAs that are specific to each of the molecular subtypes of AML. We analyzed RNA-sequencing data of 955 AML samples from three cohorts, including the BeatAML project, the Cancer Genome Atlas, and a cohort of Swedish patients to provide a comprehensive transcriptome-wide view of subtype-specific mRNA expression. We identified 729 subtype-specific mRNAs, discovered in the BeatAML project and validated in the other two cohorts. Using unique proteomics data, we also validated the presence of subtype-specific mRNAs at the protein level, yielding a rich collection of potential protein-based biomarkers for the AML community. To enable the exploration of subtype-specific mRNA expression by the broader scientific community, we provide an interactive resource to the public.

Automated summary

This study identified 729 subtype-specific mRNAs in acute myeloid leukemia (AML) through RNA sequencing data analysis and protein validation, providing a rich collection of potential protein-based biomarkers for the AML community. An interactive resource has been made available to enable broader exploration of subtype-specific mRNA expression by the scientific community.