4.6 Article

Circular RNA circEPSTI1 accelerates cervical cancer progression via miR-375/409-3P/515-5p-SLC7A11 axis

Journal

AGING-US
Volume 13, Issue 3, Pages 4663-4673

Publisher

IMPACT JOURNALS LLC
DOI: 10.18632/aging.202518

Keywords

cervical cancer; circEPSTI1; circular RNAs; miR-375; SLC7A11

Funding

  1. Hunan Provincial Groundbreaking Platform Open Fund of University of China [18K076]
  2. Hunan Provincial Health Commission [20201042]
  3. Hunan Science and Technology Innovation Project [2018SK51601]
  4. Hunan Provincial Key Subject Fund of Basic Medical Sciences
  5. University of South China
  6. Horizontal Cooperation Project of Yueyang Maternal and Child Health Hospital [2019KHX43]
  7. Hunan Provincial Health Department [B2019119]

Ask authors/readers for more resources

In this study, circEPSTI1 was found to be significantly upregulated in cervical cancer and shown to impact cell ferroptosis. The circEPSTI1-miR-375/409-3P/515-5p-SLC7A11 axis was identified to affect proliferation in cervical cancer through the ceRNA mechanism and was linked to ferroptosis. The findings suggest circEPSTI1 as a potential new biomarker for monitoring and targeting treatment in cervical cancer.
Background: Circular RNAs (circRNAs) is one kind of non-coding RNAs (ncRNAs) and exert crucial functions in biological processes and intracellular gene expression modulation. However, the biological roles and expression status of the majority of circRNAs still remain unknown in cervical cancer. Results: In this study, circEPSTI1 (hsa_circRNA_000479) was significantly upregulated in cervical cancer. We first discovered the impact of circRNA on cell ferroptosis in cervical cancer. Interestingly, circEPSTI1 attenuates the effect of ferritin which is mediated by SLC7A11 based on lipid peroxidation measurements and reduced glutathione and glutathione (GSH/GSSG) assay. Conclusions: circEPSTI1-miR-375/409-3P/515-5p-SLC7A11 axis affected the proliferation of cervical cancer via the competing endogenous RNAs (ceRNA) mechanism and was relative to ferroptosis. Our findings provided experimental evidences which revealed that circEPSTI1 might act as a new and useful biomarker for monitoring and treatment target for cervical cancer. Methods: The expression of circEPSTI1 was examined in cervical cancer cells. Then, we observed the impact of circEPSTI1 expression on the proliferation of cervical cancer by loss-of-function assays both in vivo and vitro. RIP and luciferase reporter assay revealed that circEPSTI1 sponges miR-375, miR-409-3p and miR-515-5p to upregulate SLC7A11 expression. We applied mouse xenograft experiments in mice to validate our results.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available