Journal
ADVANCED MATERIALS
Volume 33, Issue 14, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adma.202006054
Keywords
biosensors; deep learning; infrared spectroscopy; metasurfaces; nanoplasmonics
Categories
Funding
- European Research Council (ERC) [682167]
- European Union Horizon 2020 Framework Programme for Research and Innovation [777714]
- European Research Council (ERC) [682167] Funding Source: European Research Council (ERC)
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This study introduces a deep learning-augmented nanoplasmonic technique that can accurately discriminate between major classes of biomolecules without disrupting native processes.
Insights into the fascinating molecular world of biological processes are crucial for understanding diseases, developing diagnostics, and effective therapeutics. These processes are complex as they involve interactions between four major classes of biomolecules, i.e., proteins, nucleic acids, carbohydrates, and lipids, which makes it important to be able to discriminate between all these different biomolecular species. In this work, a deep learning-augmented, chemically-specific nanoplasmonic technique that enables such a feat in a label-free manner to not disrupt native processes is presented. The method uses a highly sensitive multiresonant plasmonic metasurface in a microfluidic device, which enhances infrared absorption across a broadband mid-IR spectrum and in water, despite its strongly overlapping absorption bands. The real-time format of the optofluidic method enables the collection of a vast amount of spectrotemporal data, which allows the construction of a deep neural network to discriminate accurately between all major classes of biomolecules. The capabilities of the new method are demonstrated by monitoring of a multistep bioassay containing sucrose- and nucleotides-loaded liposomes interacting with a small, lipid membrane-perforating peptide. It is envisioned that the presented technology will impact the fields of biology, bioanalytics, and pharmacology from fundamental research and disease diagnostics to drug development.
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