Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 13, Issue 10, Pages 11891-11900Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.0c21932
Keywords
carbon dioxide reduction; formate dehydrogenase; bioelectrocatalysis; oriented immobilization; metalloenzymes
Funding
- MCIU/AEI/FEDER, EU [RTI2018-095090-B-I00, PRE2019-089049]
- Fundacao para a Ciencia e Tecnologia (Portugal) [SFRH/BD/116515/2016, PTDC/BBB-EBB/2723/2014, PTDC/BII-BBF/2050/2020]
- R&D unit MOSTMICRO-ITQB [UIDB/04612/2020, UIDP/04612/2020]
- European Union's Horizon 2020 research and innovation program [810856]
- Fundação para a Ciência e a Tecnologia [PTDC/BII-BBF/2050/2020, PTDC/BBB-EBB/2723/2014, SFRH/BD/116515/2016] Funding Source: FCT
Ask authors/readers for more resources
This study investigates the covalent bonding of Desulfovibrio vulgaris Hildenborough FdhAB formate dehydrogenase to gold and graphite electrodes, showing a compact enzyme layer anchored to the electrode surface for the reduction of CO2 to formic acid via direct electron transfer. The operational stability of CO2 electroreduction to formate by direct electron transfer is demonstrated with approximately 100% Faradaic yield.
The decrease of greenhouse gases such as CO2 has become a key challenge for the human kind and the study of the electrocatalytic properties of CO2-reducing enzymes such as formate dehydrogenases is of importance for this goal. In this work, we study the covalent bonding of Desulfovibrio vulgaris Hildenborough FdhAB formate dehydrogenase to chemically modified gold and low-density graphite electrodes, using electrostatic interactions for favoring oriented immobilization of the enzyme. Electrochemical measurements show both bioelectrocatalytic oxidation of formate and reduction of CO2 by direct electron transfer (DET). Atomic force microscopy and quartz crystal microbalance characterization, as well as a comparison of direct and mediated electrocatalysis, suggest that a compact layer of formate dehydrogenase was anchored to the electrode surface with some crosslinked aggregates. Furthermore, the operational stability for CO2 electroreduction to formate by DET is shown with approximately 100% Faradaic yield.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available