Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 13, Issue 8, Pages 10321-10327Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.0c22589
Keywords
COVID-19; SARS-CoV-2; rapid antigen testing; field-effect transistor; semiconducting carbon nanotube; biosensor
Funding
- Pitt Innovation Challenge (PInCh), through the Clinical and Translational Science Institute at the University of Pittsburgh
- Defense University Research Instrumentation Program (DURIP) grant from the Office of Naval Research, ONR [N000141410765]
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This study demonstrates the use of a high-purity semiconducting single-walled carbon nanotube field-effect transistor for rapid detection of SARS-CoV-2 antigens, displaying high sensitivity and specificity.
Early diagnosis of SARS-CoV-2 infection is critical for facilitating proper containment procedures, and a rapid, sensitive antigen assay is a critical step in curbing the pandemic. In this work, we report the use of a high-purity semiconducting (sc) single-walled carbon nanotube (SWCNT)-based field-effect transistor (FET) decorated with specific binding chemistry to assess the presence of SARS-CoV-2 antigens in clinical nasopharyngeal samples. Our SWCNT FET sensors, with functionalization of the anti-SARS-CoV-2 spike protein antibody (SAb) and anti-nucleocapsid protein antibody, detected the S antigen (SAg) and N antigen (NAg), reaching a limit of detection of 0.55 fg/mL for SAg and 0.016 fg/mL for NAg in calibration samples. SAb-functionalized FET sensors also exhibited good sensing performance in discriminating positive and negative clinical samples, indicating a proof of principle for use as a rapid COVID-19 antigen diagnostic tool with high analytical sensitivity and specificity at low cost.
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