Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 13, Issue 5, Pages 5975-5988Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.0c19792
Keywords
poly(lactic-co-glycolic acid) (PLGA); tetramethylrhodamine (TRITC); mouse oocyte; preimplantation embryonic development; genetic stability; oocyte maturation
Funding
- National Research Foundation of Korea (NRF) - Korean Government [NRF-2019R1A103032888, NRF-2020R1A2C3009783]
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Through the study, it was found that only TRNPs could pass through the ZP layer around oocytes for drug delivery, and they did not negatively affect fertility or genetic stability.
The structural layers around oocytes make it difficult to deliver drugs aimed at treating infertility. In this study, we sought to identify nanoparticles (NPs) that could easily pass through zona pellucida (ZP), a special layer around oocytes, for use as a drug delivery carrier. Three types of NPs were tested: quantum dot NPs, PE-polyethylene glycol (PEG)-loaded poly(lactic-co-glycolic acid) (PLGA) NPs (PEG/ PL), and tetramethylrhodamine-loaded PLGA NPs (TRNPs). When mouse oocytes were treated with NPs, only TRNPs could fully pass through the ZP and cell membrane. To assess the effects of TRNPs on fertility and potential nanotoxicity, we performed mRNA sequencing analysis to confirm their genetic safety. We established a system to successfully internalize TRNPs into oocytes. The genetic stability and normal development of TRNP-treated oocytes and embryos were confirmed. These results imply that TRNPs can be used as a drug delivery carrier applicable to germ cells.
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