Recent Advances in Microfluidics-Based Chromatography-A Mini Review

Microfluidics-based liquid chromatography is based on the miniaturization of the different types of liquid chromatography (LC) systems (e.g., affinity, adsorption, size exclusion, ion exchange) on a microchip to perform on-chip separation of different types of analytes. On-chip chromatography finds applications in genomics, proteomics, biomarker discovery, and environmental analysis. Microfluidics-based chromatography has good reproducibility and small sample consumption. However, the on-chip chromatography fabrication techniques are often more challenging to perform than conventional LC column preparation. Different research groups have attempted to develop different techniques to fabricate microfluidics-based LC systems. In this review, we will summarize the recent advances in microfluidics-based chromatography.

Automated summary

Microfluidics-based liquid chromatography, which involves miniaturization of different LC systems on a microchip for on-chip separation, is widely used in genomics, proteomics, biomarker discovery, and environmental analysis. Despite having good reproducibility and low sample consumption, fabrication techniques for on-chip chromatography are more challenging than conventional LC column preparation.