Journal
LIFE SCIENCE ALLIANCE
Volume 4, Issue 2, Pages -Publisher
LIFE SCIENCE ALLIANCE LLC
DOI: 10.26508/lsa.202000967
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Funding
- National Institutes of Health (NIH) [R01 AI54423, R01 DK101332, R37 CA244970]
- Cellular Imaging, Comparative Medicine, Genomics & Bioinformatics, and Research Pathology Shared Resources of the Fred Hutch/University of Washington Cancer Consortium [P30 CA015704]
- Cancer Research Institute
- Debbie's Dream Foundation-American Association for Cancer Research (AACR) Gastric Cancer Research Fellowship [18-40-41-OBRI]
- Jacques Chiller Award from the Department of Microbiology, University of Washington
- Department of Veterans Affairs Merit Review Award [IBX000930]
- Department of Defense (DOD) [CA160479]
- Cancer UK Grand Challenge Award
- DOD [R37 CA244970, CA160399]
- Vanderbilt Digestive Disease Research Center [DK058404]
- Vanderbilt-Ingram Cancer Center GI SPORE [P50CA236733]
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This study reveals the significant impact of Hp infection on the progression of gastric precancerous lesions, using transgenic mice with active KRAS expression to mimic the process induced by Hp infection. Hp infection can exacerbate T-cell infiltration and macrophage polarization, leading to more metaplastic and dysplastic glands.
More than 80% of gastric cancer is attributable to stomach infection with Helicobacter pylori (Hp). Gastric preneoplastic progression involves sequential tissue changes, including loss of parietal cells, metaplasia and dysplasia. In transgenic mice, active KRAS expression recapitulates these tissue changes in the absence of Hp infection. This model provides an experimental system to investigate additional roles of Hp in preneoplastic progression, beyond its known role in initiating inflammation. Tissue histology, gene expression, the immune cell repertoire, and metaplasia and dysplasia marker expression were assessed in KRAS+ mice +/-Hp infection. Hp+/KRAS+ mice had severe T-cell infiltration and altered macrophage polarization; a different trajectory of metaplasia; more dysplastic glands; and greater proliferation of metaplastic and dysplastic glands. Eradication of Hp with antibiotics, even after onset of metaplasia, prevented or reversed these tissue phenotypes. These results suggest that gastric preneoplastic progression differs between Hp+ and Hp-cases, and that sustained Hp infection can promote the later stages of gastric preneoplastic progression.
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