4.5 Article

DMPC Phospholipid Bilayer as a Potential Interface for Human Cystatin C Oligomerization: Analysis of Protein-Liposome Interactions Using NMR Spectroscopy

Journal

MEMBRANES
Volume 11, Issue 1, Pages -

Publisher

MDPI
DOI: 10.3390/membranes11010013

Keywords

human cystatin C; NMR spectroscopy; N-15 relaxation; phospholipid; DMPC; liposome; protein– liposome interaction

Funding

  1. National Science Centre, Poland [2018/30/M/ST4/00039]
  2. Polish National Agency for Academic Exchange [PPN/PPO/2018/1/00071/DEC/1]
  3. [GA731019]
  4. [H2020-EU.1.4.1.2]
  5. [H2020-INFRAIA-2016-2017]

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Studying the mechanisms behind amyloid-based diseases can help identify future treatment targets, but the complexity and interactions of proteins responsible for fibril formation make it a challenging and time-consuming task. Human cystatin C, a cysteine-protease inhibitor, tends to form fibrils under certain stimuli, potentially regulated by cellular components with the cell membrane playing a key role in the oligomerization pathway. The use of phospholipid liposomes as membrane mimetics provides a promising alternative for studying protein-mimetic interactions.
Studies revolving around mechanisms responsible for the development of amyloid-based diseases lay the foundations for the recognition of molecular targets of future to-be-developed treatments. However, the vast number of peptides and proteins known to be responsible for fibril formation, combined with their complexity and complexity of their interactions with various cellular components, renders this task extremely difficult and time-consuming. One of these proteins, human cystatin C (hCC), is a well-known and studied cysteine-protease inhibitor. While being a monomer in physiological conditions, under the necessary stimulus-usually a mutation, it tends to form fibrils, which later participate in the disease development. This process can potentially be regulated (in several ways) by many cellular components and it is being hypothesized that the cell membrane might play a key role in the oligomerization pathway. Studies involving cell membranes pose several difficulties; therefore, an alternative in the form of membrane mimetics is a very attractive solution. Here, we would like to present the first study on hCC oligomerization under the influence of phospholipid liposomes, acting as a membrane mimetic. The protein-mimetic interactions are studied utilizing circular dichroism, nuclear magnetic resonance, and size exclusion chromatography.

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