Journal
BIOSENSORS-BASEL
Volume 11, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/bios11010021
Keywords
l-phenylalaine; surface plasmon resonance; sensor; molecular imprinting technology; detection
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The study designed a surface plasmon resonance sensor for detecting L-phenylalaine using molecular imprinting technology, achieving rapid, sensitive, and selective detection. Characterization and kinetic studies were conducted, revealing good detection limits and response time in various experiments.
In this study, we designed a simple, rapid, sensitive and selective surface plasmon resonance (SPR) sensor for detection of L-phenylalaine by utilizing molecular imprinting technology. l-phenylalanine imprinted and non-imprinted poly(2-hydroxyethyl methacrylate-methacryloyl-l-phenylalanine) polymeric films were synthesized onto SPR chip surfaces using ultraviolet polymerization. l-phenyalanine imprinted and non-imprinted SPR sensors were characterized by using contact angle, atomic force microscopy and ellipsometry. After characterization studies, kinetic studies were carried out in the concentration range of 5.0-400.0 mu M. The limit of detection and quantification were obtained as 0.0085 and 0.0285 mu M, respectively. The response time for the test including equilibration, adsorption and desorption was approximately 9 min. The selectivity studies of the l-phenylalanine imprinted SPR sensor was performed in the presence of d-phenylalanine and l-tryptophan. Validation studies were carried out via enzyme-linked immunosorbent analysis technique in order to demonstrate the applicability and superiority of the l-phenylalanine imprinted SPR sensor.
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