Streamlined copper defenses make Bordetella pertussis reliant on custom-made operon

Copper is both essential and toxic to living beings, which tightly controls its intracellular concentration. At the host-pathogen interface, copper is used by phagocytic cells to kill invading microorganisms. We investigated copper homeostasis in Bordetella pertussis, which lives in the human respiratory mucosa and has no environmental reservoir. B. pertussis has considerably streamlined copper homeostasis mechanisms relative to other Gram-negative bacteria. Its single remaining defense line consists of a metallochaperone diverted for copper passivation, CopZ, and two peroxide detoxification enzymes, PrxGrx and GorB, which together fight stresses encountered in phagocytic cells. Those proteins are encoded by an original, composite operon assembled in an environmental ancestor, which is under sensitive control by copper. This system appears to contribute to persistent infection in the nasal cavity of B. pertussis-infected mice. Combining responses to co-occurring stresses in a tailored operon reveals a strategy adopted by a host-restricted pathogen to optimize survival at minimal energy expenditure. Alex Rivera-Millot et al. investigate copper homeostasis in the whooping cough agent Bordetella pertussis, which has a single copper defense mechanism via a metallochaperone diverted for copper passivation and two peroxide detoxification enzymes. This study demonstrates that copper up-regulates the copZ-prxgrx-gorB operon in macrophages, and this system appears to contribute to persistent infection in the nasal cavity of B. pertussis-infected mice. This study brings insight into strategies aimed to optimize survival of a host-restricted pathogen.

Automated summary

The study investigates copper homeostasis in Bordetella pertussis, a whooping cough agent with a single copper defense mechanism via a metallochaperone and two peroxide detoxification enzymes. The study demonstrates that copper up-regulates the copZ-prxgrx-gorB operon in macrophages, contributing to persistent infection in the nasal cavity of infected mice. This highlights the strategy adopted by a host-restricted pathogen to optimize survival.