Journal
BIOMEDICINES
Volume 8, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/biomedicines8120594
Keywords
kefir; lactic acid bacteria; probiotics; adherence; cancer cells; apoptosis; flow cytometry
Categories
Funding
- project: Research Infrastructure on Food Bioprocessing Development and Innovation Exploitation-Food Innovation RI [MIS 5027222]
- project: OPENSCREEN-GR: An Open-Access Research Infrastructure of Target-Based Screening Technologies and Chemical Biology for Human and Animal Health, Agriculture and Environment [MIS 5002691]
- European Union (European Regional Development Fund)
- Operational Program Competitiveness, Entrepreneurship and Innovation (NSRF 2014-2020)
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Kappa efir is a rich source of potentially probiotic bacteria. In the present study, firstly, in vitro screening for probiotic characteristics of ten lactic acid bacteria (LAB) isolated from kefir grains was performed. Strain AGR 4 was selected for further studies. Molecular characterization of strain AGR 4, confirmed that AGR 4 belongs to the Lactobacillus paracasei (reclassified to Lacticaseibacillus paracasei subsp. paracasei) species. Further testing revealed that L. paracasei AGR 4 displayed adhesion capacity on human adenocarcinoma cells, HT-29, similar to that of the reference strain, L. casei ATCC 393. In addition, the novel strain exerted significant time- and dose-dependent antiproliferative activity against HT-29 cells and human melanoma cell line, A375, as demonstrated by the sulforhodamine B cytotoxicity assay. Flow cytometry analysis was employed to investigate the mechanism of cellular death; however, it was found that AGR 4 did not act by inducing cell cycle arrest and/or apoptotic cell death. Taken together, these findings promote the probiotic character of the newly isolated strain L. paracasei AGR 4, while further studies are needed for the detailed description of its biological properties.
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