Journal
BIOMEDICINES
Volume 8, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/biomedicines8120563
Keywords
three-dimensional printing; PET/CT imaging; rectal drug delivery; ulcerative colitis; TNBS rat model; M3dimaker; 3D printed drug products; personalized medicines and pharmaceuticals
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Funding
- Xunta de Galicia [GRC2013/015, GPC2017/015]
- Instituto de Salud Carlos III (Juan Rodes research grant) [JR18/00014]
- IDIS (Health Research Institute of Santiago de Compostela)
- [RYC-2015/17430]
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The aim of this study was to fabricate novel self-supporting tacrolimus suppositories using semisolid extrusion 3-dimensional printing (3DP) and to investigate their efficacy in an experimental model of inflammatory bowel disease. Blends of Gelucire 44/14 and coconut oil were employed as lipid excipients to obtain suppository formulations with self-emulsifying properties, which were then tested in a TNBS (2,4,6-trinitrobenzenesulfonic acid) induced rat colitis model. Disease activity was monitored using PET/CT medical imaging; maximum standardized uptake values (SUVmax), a measure of tissue radiotracer accumulation rate, together with body weight changes and histological assessments, were used as inflammatory indices to monitor treatment efficacy. Following tacrolimus treatment, a significant reduction in SUVmax was observed on days 7 and 10 in the rat colon sections compared to non-treated animals. Histological analysis using Nancy index confirmed disease remission. Moreover, statistical analysis showed a positive correlation (R-2 = 71.48%) between SUVmax values and weight changes over time. Overall, this study demonstrates the effectiveness of 3D printed tacrolimus suppositories to ameliorate colitis and highlights the utility of non-invasive PET/CT imaging to evaluate new therapies in the preclinical area.
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