A Nonenzymatic Hydrogen Peroxide Electrochemical Sensing and Application in Cancer Diagnosis

The diagnosis of malignant tumors is essential for informing clinical decisions regarding therapeutic options. Current imaging and pathological diagnostic methods do not provide quantitative molecular information that is important in tumor identification. Moreover, pathological tissue analysis is dependent on unevenly distributed pathological features. The tumor microenvironment has been documented to have hydrogen peroxide (H2O2). This study presents a biologically sensitive and efficient H2O2 electrochemical sensor based on PtNi nanoparticle-doped N-reduced graphene oxide (PtNi-N-rGO) with a low detection limit (2.8 x 10(-9) m), a fast response time (<6 s) and desirable anti-interference characteristics. Herein, H2O2 is used as molecular biomarker. The sensor successfully captures H2O2 from cancer cells. In addition, it efficiently detects tumor tissues, adjacent tissues, and normal tissues. This study demonstrates the H2O2 sensor potential to rapidly detect tumor tissues. This technique provides a complementary method for pathological tumor diagnosis that is independent of the traditional pathology labs.

Automated summary

The study presents a biologically sensitive and efficient H2O2 electrochemical sensor based on PtNi nanoparticle-doped N-reduced graphene oxide, which successfully captures H2O2 from cancer cells and efficiently detects tumor tissues. This technique provides a complementary method for pathological tumor diagnosis independent of traditional pathology labs.