Somatic Embryogenesis in Centaurium erythraea Rafn-Current Status and Perspectives: A Review

Centaurium erythraea (centaury) is a traditionally used medicinal plant, with a spectrum of secondary metabolites with confirmed healing properties. Centaury is an emerging model in plant developmental biology due to its vigorous regenerative potential and great developmental plasticity when cultured in vitro. Hereby, we review nearly two decades of research on somatic embryogenesis (SE) in centaury. During SE, somatic cells are induced by suitable culture conditions to express their totipotency, acquire embryogenic characteristics, and eventually give rise to somatic embryos. When SE is initiated from centaury root explants, the process occurs spontaneously (on hormone-free medium), directly (without the callusing phase), and the somatic embryos are of unicellular origin. SE from leaf explants has to be induced by plant growth regulators and is indirect (preceded by callusing). Histological observations and culture conditions are compared in these two systems. The changes in antioxidative enzymes were followed during SE from the leaf explants. Special focus is given to the role of arabinogalactan proteins during SE, which were analyzed using a variety of approaches. The newest and preliminary results, including centaury transcriptome, novel potential SE markers, and novel types of arabinogalactan proteins, are discussed as perspectives of centaury research.

Automated summary

The review provides an overview of nearly two decades of research on somatic embryogenesis in Centaurium erythraea, highlighting differences between initiation from root explants and leaf explants, as well as the roles of antioxidative enzymes and arabinogalactan proteins during the process. The discussion also includes the latest findings on centaury transcriptome, potential SE markers, and novel types of arabinogalactan proteins, pointing towards future directions in centaury research.