Journal
FRONTIERS IN ECOLOGY AND EVOLUTION
Volume 8, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fevo.2020.618007
Keywords
neurogenesis; single-cell RNAseq; annelid; cell type; differentiation trajectory; pseudotime; RNA velocity; gene regulatory network
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Funding
- Sigma Xi [G2018031596148406]
- Clark University - Lise Ann and Leo E. Beavers' fund
- Clark University - Fechter-Stansbury fund
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This study mapped larval cell types in the annelid Capitella teleta using single-cell transcriptomics, identifying 8 unique cell clusters and delving deeper into neural and neurosecretory cells. Analysis revealed distinct neural differentiation trajectories, providing valuable insights into neurogenesis in annelids.
Evolution and diversification of cell types has contributed to animal evolution. However, gene regulatory mechanisms underlying cell fate acquisition during development remains largely uncharacterized in spiralians. Here we use a whole-organism, single-cell transcriptomic approach to map larval cell types in the annelid Capitella teleta at 24- and 48-h post gastrulation (stages 4 and 5). We identified eight unique cell clusters (undifferentiated precursors, ectoderm, muscle, ciliary-band, gut, neurons, neurosecretory cells, and protonephridia), thus helping to identify uncharacterized molecular signatures such as previously unknown neurosecretory cell markers in C. teleta. Analysis of coregulatory programs in individual clusters revealed gene interactions that can be used for comparisons of cell types across taxa. We examined the neural and neurosecretory clusters more deeply and characterized a differentiation trajectory starting from dividing precursors to neurons using Monocle3 and velocyto. Pseudotime analysis along this trajectory identified temporally-distinct cell states undergoing progressive gene expression changes over time. Our data revealed two potentially distinct neural differentiation trajectories including an early trajectory for brain neurosecretory cells. This work provides a valuable resource for future functional investigations to better understanding neurogenesis and the transitions from neural precursors to neurons in an annelid.
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