Journal
FRONTIERS IN MOLECULAR BIOSCIENCES
Volume 7, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmolb.2020.627848
Keywords
CRISPR; split-dCas9; light-inducible; bladder cancer; logic gate
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Funding
- National Key R&D Program of China [2019YFA0906003]
- National Natural Science Foundation of China [82073364]
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Optogenetic systems are increasingly explored in the field of biomedicine. This study utilized AND logic gates in light-inducible genetic circuits to specifically inhibit cancer cells. The activated logic gate led to the expression of proteins that could inhibit tumor cell function, offering a potential therapeutic strategy for future bladder cancer.
Optogenetic systems have been increasingly investigated in the field of biomedicine. Previous studies had found the inhibitory effect of the light-inducible genetic circuits on cancer cell growth. In our study, we applied an AND logic gates to the light-inducible genetic circuits to inhibit the cancer cells more specifically. The circuit would only be activated in the presence of both the human telomerase reverse transcriptase (hTERT) and the human uroplakin II (hUPII) promoter. The activated logic gate led to the expression of the p53 or E-cadherin protein, which could inhibit the biological function of tumor cells. In addition, we split the dCas9 protein to reduce the size of the synthetic circuit compared to the full-length dCas9. This light-inducible system provides a potential therapeutic strategy for future bladder cancer.
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