Journal
MICROORGANISMS
Volume 9, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/microorganisms9010144
Keywords
conidia; homeobox domain; RNA-sequencing analysis; transcription factor; sterigmatocystin; Aspergillus nidulans
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Funding
- National Research Foundation of Korea (NRF) - Korean Government [NRF-2020R1C1C1004473]
- KRIBB Research Initiative Program
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HbxB in Aspergillus nidulans functions as a key transcription factor regulating trehalose biosynthesis, stress tolerance, beta-glucan degradation, and sterigmatocystin production in conidia. Its deletion affects gene expression associated with these processes, leading to increased sensitivity to UV stress and alterations in beta-glucan levels and sterigmatocystin production.
Homeobox transcription factors are conserved in eukaryotes and act as multi-functional transcription factors in filamentous fungi. Previously, it was demonstrated that HbxB governs fungal development and spore viability in Aspergillus nidulans. Here, the role of HbxB in A. nidulans was further characterized. RNA-sequencing revealed that HbxB affects the transcriptomic levels of genes associated with trehalose biosynthesis and response to thermal, oxidative, and radiation stresses in asexual spores called conidia. A phenotypic analysis found that hbxB deletion mutant conidia were more sensitive to ultraviolet stress. The loss of hbxB increased the mRNA expression of genes associated with beta-glucan degradation and decreased the amount of beta-glucan in conidia. In addition, hbxB deletion affected the expression of the sterigmatocystin gene cluster and the amount of sterigmatocystin. Overall, these results indicated that HbxB is a key transcription factor regulating trehalose biosynthesis, stress tolerance, beta-glucan degradation, and sterigmatocystin production in A. nidulans conidia.
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