Journal
ANTIOXIDANTS
Volume 10, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/antiox10010101
Keywords
microRNAs; visceral adipose tissue; visceral adipocytes; hyperglycemia; diabetes; impaired fasting glucose; sex differences; oxidative stress; inflammation; insulin signaling
Funding
- Polish National Science Center [2015/17/B/NZ7/03019]
- Medical University of Lodz [503/1-159-01/503-21-001]
- Polish Society of Metabolic Disorders
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The study found that high blood sugar may affect the expression of specific miRNAs in visceral adipose tissue, with these miRNAs significantly upregulated in IFG and T2DM patients, positively correlated with FPG and HbA1c, but not associated with WHR/BMI, potentially related to low-grade chronic inflammation and oxidative stress.
Hypertrophic and hypoxic visceral adipose tissue (VAT) secretes proinflammatory cytokines promoting insulin resistance (IR), prediabetes and type 2 diabetes (T2DM) microRNAs (miRNAs) are markers of metabolic disorders regulating genes critical for e.g., inflammation, glucose metabolism, and antioxidant defense, with raising diagnostic value. The aim of the current study was to evaluate whether hyperglycemia is able to affect the expression of selected miRNAs in VAT of prediabetic (IFG) and diabetic (T2DM) patients vs. normoglycemic (NG) subjects using qPCR. Statistical analyses suggested that miRNAs expression could be sex-dependent. Thus, we determined 15 miRNAs as differentially expressed (DE) among NG, T2DM, IFG females (miR-10a-5p, let-7d-5p, miR-532-5p, miR-127-3p, miR-125b-5p, let-7a-5p, let-7e-5p, miR-199a-3p, miR-365a-3p, miR-99a-5p, miR-100-5p, miR-342-3p, miR-146b-5p, miR-204-5p, miR-409-3p). Majority of significantly changed miRNAs was similarly upregulated in VAT of female T2DM and IFG patients in comparison to NG subjects, positively correlated with FPG and HbA1c, yet, uncorrelated with WHR/BMI. Enrichment analyses indicated involvement of 11 top DE miRNAs in oxidative stress, inflammation and insulin signaling. Those miRNAs expression changes could be possibly associated with low-grade chronic inflammation and oxidative stress in VAT of hyperglycemic subjects.
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