Journal
BIOMOLECULES
Volume 10, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/biom10111581
Keywords
Huntington's disease; huntingtin protein (Htt); Blm10/PA200-proteasomes; degradation; soluble N-Htt; mutant N-Htt aggregates
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Funding
- University of Debrecen, Debrecen, Hungary
- Bridging Fund
- International Education Office, University of Debrecen, Debrecen, Hungary
- Tempus Foundation, Stipendium Hungaricum
- National Natural Science Foundation of China [81901284]
- Shanghai Science and Technology [18ZR1421700]
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The Blm10/PA200 family of proteasome activators modulates the peptidase activity of the core particle (20S CP). They participate in opening the 20S CP gate, thus facilitating the degradation of unstructured proteins such as tau and Dnm1 in a ubiquitin- and ATP-independent manner. Furthermore, PA200 also participates in the degradation of acetylated histones. In our study, we use a combination of yeast and human cell systems to investigate the role of Blm10/PA200 in the degradation of N-terminal Huntingtin fragments (N-Htt). We demonstrate that the human PA200 binds to N-Htt. The loss of Blm10 in yeast or PA200 in human cells results in increased mutant N-Htt aggregate formation and elevated cellular toxicity. Furthermore, Blm10 in vitro accelerates the proteasomal degradation of soluble N-Htt. Collectively, our data suggest N-Htt as a new substrate for Blm10/PA200-proteasomes and point to new approaches in Huntington's disease (HD) research.
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