Journal
NATURE BIOMEDICAL ENGINEERING
Volume 4, Issue 12, Pages 1159-1167Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41551-020-00654-0
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Funding
- Institute for Basic Science [IBS-R026-D1]
- US NIH [R01CA229777, R21DA049577, U01CA233360]
- MGH Scholar Fund
- US DOD [W81XWH1910199, W81XWH1910194]
- U.S. Department of Defense (DOD) [W81XWH1910199, W81XWH1910194] Funding Source: U.S. Department of Defense (DOD)
- National Research Foundation of Korea [IBS-R026-D1-2020-A00, 4120200213582] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The diagnosis of severe acute respiratory syndrome 2 (SARS-CoV-2) infection by quantitative PCR with reverse transcription (RT-qPCR) typically involves bulky instrumentation in centralized laboratories and an assay time of 1-2 h. Here, we show that SARS-CoV-2 RNA can be detected in 17 min via a portable device integrating reverse transcription, fast thermocycling (via plasmonic heating through magneto-plasmonic nanoparticles) and in situ fluorescence detection following magnetic clearance of the nanoparticles. The device correctly classified all nasopharyngeal, oropharyngeal and sputum samples from 75 patients with COVID-19 and 75 healthy controls, with good concordance in fluorescence intensity with standard RT-qPCR (Pearson coefficients > 0.7 for the N1, N2 and RPP30 genes). Fast, portable and automated nucleic acid detection should facilitate testing at the point of care.
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