Journal
PHARMACEUTICS
Volume 13, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/pharmaceutics13010059
Keywords
niosome; gemcitabine; cisplatin; heating method; lung cancer; aerosol output
Categories
Funding
- Ministry of Higher Education, Malaysia under LRGS NanoMITe project [RU029-2014/5526306]
- Graduate Research Fellowship (GRF) from the Universiti Putra Malaysia
Ask authors/readers for more resources
The study developed a niosome formulation NGC with low toxicity and good stability for lung cancer treatment. In vitro experiments showed that NGC has reduced cytotoxic effects on normal lung and lung cancer cells compared to conventional drugs.
Gemcitabine (Gem) and cisplatin (Cis) are currently being used for lung cancer treatment, but they are highly toxic in high dosages. This research aimed to develop a niosome formulation containing a low-dosage Gem and Cis (NGC), as an alternative formulation for lung cancer treatment. NGC was prepared using a very simple heating method and was further optimized by D-optimal mixture design. The optimum NGC formulation with particle size, polydispersity index (PDI), and zeta potential of 166.45 nm, 0.16, and -15.28 mV, respectively, was obtained and remained stable at 27 degrees C with no phase separation for up to 90 days. The aerosol output was 96.22%, which indicates its suitability as aerosolized formulation. An in vitro drug release study using the dialysis bag diffusion technique showed controlled release for both drugs up to 24 h penetration. A cytotoxicity study against normal lung (MRC5) and lung cancer (A549) cell lines was investigated. The results showed that the optimized NGC had reduced cytotoxicity effects against both MRC5 and A549 when compared with the control (Gem + Cis alone) from very toxic (IC50 < 1.56 mu g/mL) to weakly toxic (IC50 280.00 mu g/mL) and moderately toxic (IC50 = 46.00 mu g/mL), respectively, after 72 h of treatment. These findings revealed that the optimized NGC has excellent potential and is a promising prospect in aerosolized delivery systems to treat lung cancer that warrants further investigation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available