Journal
FRONTIERS IN ONCOLOGY
Volume 10, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fonc.2020.614876
Keywords
humanized mice; immuno-oncology; Epstein-Barr Virus (EBV); immune checkpoint inhibition (ICI); post-transplant lymphoproliferative disease (PTLD); lymphoma; PD-1; pembrolizumab
Categories
Funding
- German Center for Infections Research [DZIF-TTU07.803, DZIF-TTU07.805]
- Jackson Laboratory
- Else Kroner-Fresenius/Fortra
- RegSci Ph.D. fellowship
- German Ministry of Education and Research (BMBF), DLR project management (e:Med) grants [01ZX1710A, 01ZX1608A]
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PTLD is a common malignancy after organ transplantation, often B cell neoplasias carrying EBV. Therapeutic approaches include reducing immunosuppression and immunotherapies targeting CD20. Immune checkpoint inhibition for EBV+ PTLD is not established due to risks of organ rejection and GVHD.
Post-transplant lymphoproliferative disorder (PTLD) is one of the most common malignancies after solid organ or allogeneic stem cell transplantation. Most PTLD cases are B cell neoplasias carrying Epstein-Barr virus (EBV). A therapeutic approach is reduction of immunosuppression to allow T cells to develop and combat EBV. If this is not effective, approaches include immunotherapies such as monoclonal antibodies targeting CD20 and adoptive T cells. Immune checkpoint inhibition (ICI) to treat EBV+ PTLD was not established clinically due to the risks of organ rejection and graft-versus-host disease. Previously, blockade of the programmed death receptor (PD)-1 by a monoclonal antibody (mAb) during ex vivo infection of mononuclear cells with the EBV/M81(+) strain showed lower xenografted lymphoma development in mice. Subsequently, fully humanized mice infected with the EBV/B95-8 strain and treated in vivo with a PD-1 blocking mAb showed aggravation of PTLD and lymphoma development. Here, we evaluated vis-a-vis in fully humanized mice after EBV/B95-8 or EBV/M81 infections the effects of a clinically used PD-1 blocker. Fifteen to 17 weeks after human CD34(+) stem cell transplantation, Nod.Rag.Gamma mice were infected with two types of EBV laboratory strains expressing firefly luciferase. Dynamic optical imaging analyses showed systemic EBV infections and this triggered vigorous human CD8(+) T cell expansion. Pembrolizumab administered from 2 to 5 weeks post-infections significantly aggravated EBV systemic spread and, for the M81 model, significantly increased the mortality of mice. ICI promoted Ki67(+)CD30(+)CD20(+)EBER(+)PD-L1(+) PTLD with central nervous system (CNS) involvement, mirroring EBV+ CNS PTLD in humans. PD-1 blockade was associated with lower frequencies of circulating T cells in blood and with a profound collapse of CD4(+) T cells in lymphatic tissues. Mice treated with pembrolizumab showed an escalation of exhausted T cells expressing TIM-3, and LAG-3 in tissues, higher levels of several human cytokines in plasma and high densities of FoxP3(+) regulatory CD4(+) and CD8(+) T cells in the tumor microenvironment. We conclude that PD-1 blockade during acute EBV infections driving strong CD8(+) T cell priming decompensates T cell development towards immunosuppression. Given the variety of preclinical models available, our models conferred a cautionary note indicating that PD-1 blockade aggravated the progression of EBV+ PTLD.
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