Journal
CELLS
Volume 10, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/cells10010102
Keywords
mast cell; MRGPRX2; pseudo-allergy; Substance P; TSLP; skin
Categories
Funding
- Deutsche Forschungsgemeinschaft DFG [BA-3769/4-1]
- CSC
- ECARF (European Center for Allergy Research Foundation)
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TSLP is a novel factor that primes human skin MCs to support granule discharge, selectively cooperating with MRGPRX2 without impacting other pathways. TSLP prolongs granule opening by activating STAT5 and JNK in skin MCs, leading to reinforced degranulation.
Thymic stromal lymphopoietin (TSLP) is released by epithelial cells following disturbed homeostasis to act as alarmin and driver of Th2-immunity. Aberrant TSLP expression is a hallmark of atopic diseases, including atopic dermatitis (AD). Mast cells (MCs) are overabundant in AD lesions and show signs of degranulation, but it remains unknown whether TSLP contributes to granule discharge. Degranulation of skin MCs proceeds via two major routes, i.e., Fc epsilon RI-dependent (allergic) and MRGPRX2-mediated (pseudo-allergic/neurogenic). Evidence is accumulating that MRGPRX2 may be crucial in the context of skin diseases, including eczema. The current study reveals TSLP as a novel priming factor of human skin MCs. Interestingly, TSLP selectively cooperates with MRGPRX2 to support granule discharge, while it does not impact spontaneous or Fc epsilon RI-driven exocytosis. TSLP-assisted histamine liberation triggered by compound 48/80 or Substance P, two canonical MRGPRX2 agonists, was accompanied by an increase in CD107a+ cells (a MC activation marker). The latter process was less potent, however, and detectable only at the later of two time points, suggesting TSLP may prolong opening of the granules. Mechanistically, TSLP elicited phosphorylation of STAT5 and JNK in skin MCs and the reinforced degranulation critically depended on STAT5 activity, while JNK had a contributory role. Results from pharmacological inhibition were confirmed by RNA-interference, whereby silencing of STAT5 completely abolished the priming effect of TSLP on MRGPRX2-mediated degranulation. Collectively, TSLP is the first factor to favor MRGPRX2- over Fc epsilon RI-triggered MC activation. The relevance of TSLP, MCs and MRGPRX2 to pruritis and atopic skin pathology indicates broad repercussions of the identified connection.
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