4.5 Article

Quantitative image analysis of microbial communities with BiofilmQ

Journal

NATURE MICROBIOLOGY
Volume 6, Issue 2, Pages 151-+

Publisher

NATURE PORTFOLIO
DOI: 10.1038/s41564-020-00817-4

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Funding

  1. Studienstiftung des Deutschen Volkes
  2. Max Planck Society
  3. European Research Council [StG-716734]
  4. Human Frontier Science Program [CDA00084/2015-C]
  5. Deutsche Forschungsgemeinschaft [SFB 987]
  6. Bundesministerium fur Bildung und Forschung (TARGET-Biofilms)
  7. Behrens-Weise-Stiftung
  8. Minna James Heineman Foundation
  9. Joachim Herz Stiftung

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Biofilms are a common form of microbial life on Earth, with phenotypic and genotypic variations occurring in three-dimensional space and time, requiring microscopy and quantitative image analysis for elucidation. BiofilmQ is a comprehensive image cytometry software tool that enables visualization, quantification, and analysis of biofilm properties, providing insights into their structure and function.
Biofilms are microbial communities that represent a highly abundant form of microbial life on Earth. Inside biofilms, phenotypic and genotypic variations occur in three-dimensional space and time; microscopy and quantitative image analysis are therefore crucial for elucidating their functions. Here, we present BiofilmQ-a comprehensive image cytometry software tool for the automated and high-throughput quantification, analysis and visualization of numerous biofilm-internal and whole-biofilm properties in three-dimensional space and time. BiofilmQ is an image cytometry software tool that enables the visualization, quantification and analysis of biofilm properties, providing insights into their structure and function.

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