Journal
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
Volume 19, Issue -, Pages 35-46Publisher
CELL PRESS
DOI: 10.1016/j.omtm.2020.08.013
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Funding
- National Science and Technology Major Projects of New Drugs [2018ZX09201018-013]
- National Science and Technology Major Project for Infectious Diseases Control [2017ZX10203206-004]
- National Natural Science Foundation of China [81101728]
- Sichuan Regional Innovation Cooperation Project [20QYCX0100]
- Innovation Spark Project of Sichuan University [2018SCUH0084]
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To ensure the high purity and biological activity of the adenovirus vector to be used for clinical applications, a stable and linearly scalable preparation method is highly imperative. During the adenovirus-harvesting process, the Triton X-100-based lysis method possesses the advantages of higher efficiency as well as easier linearization and amplification. Most Triton X100 can be removed from the adenovirus sample by chromatographic purification. However, there is no report that a small amount of residual Triton X-100, present in adenovirus sample, can affect the particle integrity, infectivity, and structure of adenoviruses. Here, we found that although residual Triton X-100 affected the short-term stability, purity, infectivity, and structure of adenoviruses at 37 degrees C, it did not hamper these properties of adenoviruses at 4 degrees C. This study suggests that although the Triton X-100-based lysis method is a simple, efficient, and easy-to-scale process for lysing host cells to release the adenovirus, the storage conditions of adenovirus products must be taken into consideration.
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