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Emerging Technologies for Genome-Wide Profiling of DNA Breakage

Journal

FRONTIERS IN GENETICS
Volume 11, Issue -, Pages -

Publisher

FRONTIERS MEDIA SA
DOI: 10.3389/fgene.2020.610386

Keywords

genome instability; DNA damage; double strand break (DSB); single strand break (SSB); neurodegeneration; neurodegenerative disease; aging

Funding

  1. National Institute of Health [R21 NS102829 ZZ]
  2. National Science Foundation of China [31671382]
  3. Scientific Research Funds of Huaqiao University

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Genome instability is a known feature of various human diseases, with new techniques for detecting DNA damage emerging recently. These tools show promising potential for studying neurodegenerative diseases.
Genome instability is associated with myriad human diseases and is a well-known feature of both cancer and neurodegenerative disease. Until recently, the ability to assess DNA damage-the principal driver of genome instability-was limited to relatively imprecise methods or restricted to studying predefined genomic regions. Recently, new techniques for detecting DNA double strand breaks (DSBs) and single strand breaks (SSBs) with next-generation sequencing on a genome-wide scale with single nucleotide resolution have emerged. With these new tools, efforts are underway to define the breakome in normal aging and disease. Here, we compare the relative strengths and weaknesses of these technologies and their potential application to studying neurodegenerative diseases.

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