Journal
ACS CENTRAL SCIENCE
Volume 6, Issue 12, Pages 2250-2258Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acscentsci.0c00763
Keywords
-
Categories
Funding
- KIST
- Pioneer Research Center Program [2014M3C1A3054141]
- National Research Foundation of Korea - Korea government (MSIT) [2020R1A2C2008213]
- Bio & Medical Technology Development Program of the National Research Foundation (NRF) - Korean government (MSIT) [2020M3E5E2037598]
- National Research Foundation of Korea [2020M3E5E2037598, 2020R1A2C2008213] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
A proper intracellular delivery method with target tissue specificity is critical to utilize the full potential of therapeutic molecules including siRNAs while minimizing their side effects. Herein, we prepare four small-sized DNA tetrahedrons (sTds) by self-assembly of different sugar backbone-modified oligonucleotides and screened them to develop a platform for kidney-targeted cytosolic delivery of siRNA. An in vivo biodistribution study revealed the kidney-specific accumulation of mirror DNA tetrahedron (L-sTd). Low opsonization of L-sTd in serum appeared to avoid liver clearance and keep its size small enough to be filtered through the glomerular basement membrane (GBM). After GBM filtration, L-sTd could be delivered into tubular cells by endocytosis. The kidney preference and the tubular cell uptake property of the mirror DNA nanostructure could be successfully harnessed for kidney-targeted intracellular delivery of p53 siRNA to treat acute kidney injury (AKI) in mice. Therefore, L-sTd could be a promising platform for kidney-targeted cytosolic delivery of siRNA to treat renal diseases.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available