Journal
APPLIED SCIENCES-BASEL
Volume 10, Issue 24, Pages -Publisher
MDPI
DOI: 10.3390/app10249040
Keywords
chorionic mesenchymal stem cells; immunomodulation; lymphocytes
Categories
Funding
- Slovak Agency for Research and Development [APVV-17-0278]
- Cultural Educational Grant Agency of the Ministry of Education, Science, Research and Sport of the Slovak Republic [KEGA 023TUKE-4/2020, KEGA 041TUKE-4/2019, IMTS 26220220185, ITMS2014+ 313011D103, BE 81 3DP TISSUE]
- incentives for research and development Stimuly BE 81 3DP TISSUE
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Mesenchymal stem cells (MSCs) represent a population of adherent cells that can be isolated from multiple adult tissues. MSCs have immunomodulatory capacity and the ability to differentiate into many cell lines. Research study examines the immunomodulatory properties of MSCs isolated from chorion (CMSCs). Following the stimulation process, it was found that MSCs are capable of immunomodulatory action via the release of bioactive molecules as well as through direct contact with the immune cells. Immunomodulatory potential of the CMSCs was analyzed by modifying proliferative capacity of mitogen-activated lymphocytes. CMSCs and lymphocytes were tested in cell-to-cell contact. Lymphocytes were stained with carboxyfluorescein diacetate succinimidyl ester. Inhibition of the proliferation of activated lymphocytes was observed. Following the co-cultivation, the expression of markers involved in the immune response modulation was assessed. Afterwards, an increase in CMSCs expression of IL-10 was detected. Following the co-cultivation with activated lymphocyte, adhesion molecules CD54 and CD44 in the CMSCs increased. An increase of CD54 expression was observed. The properties of CMSCs, adherence and differentiation ability, were confirmed. The phenotype of CMSCs CD105+, CD90+, CD73+, CD44+, CD29+, CD45-, CD34-, CD54+ was characterized. It was demonstrated that chorion-derived MSCs have important immunomodulatory effects.
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