Journal
AMB EXPRESS
Volume 10, Issue 1, Pages -Publisher
SPRINGER
DOI: 10.1186/s13568-020-01163-8
Keywords
PCV3; Capsid protein; Expression; High repetitive; Arginine; Obstacle
Categories
Funding
- Education Department of Liaoning Province [LSNFW201710, LSNYB201612]
- National Key Program for Infectious Disease of China [2018ZX10732401-001, 2018ZX10101002-002]
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Porcine circovirus type 3 (PCV3) is a novel circovirus identified in sows with PDNS-like clinical signs and reproductive failure. The capsid protein (CAP) of PCV3 is expected to be an effective vaccine candidate. Here, we expressed the original capsid protein, truncated capsid protein without anterior highly repetitive arginine (Delta CAP) and their codon-optimized counterparts in E. coli. These results showed that lots of repeated arginine could severely lower the expression of PCV3 capsid protein in E. coli. At the same time, the recombined truncated PCV3 capsid protein forms typic virions. The efficient expression of capsid protein is expected to serve the development of PCV3 vaccines and other studies of PCV3 capsid protein.
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