Journal
GENES
Volume 12, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/genes12020160
Keywords
Canis lupus familiaris; dog; skin disease; cutaneous T-cell lymphoma; lupus erythematosus; cytotoxic dermatitis; RNA sequencing; FFPE tissue; transcriptome
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Funding
- European Society of Veterinary Dermatology (ESVD Major Grant 2018)
- Berne University Research Foundation [18/2018]
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This study investigated transcriptional differences between canine CETL and IMD, aiming to establish discriminatory markers for further research. The research found distinct gene expression profiles in CETL and IMD, with differentially expressed genes potentially serving as discriminatory markers but needing further validation.
Canine cutaneous epitheliotropic T-cell lymphoma (CETL) and immune-mediated T-cell predominant dermatoses (IMD) share several clinical and histopathological features, but differ substantially in prognosis. The discrimination of ambiguous cases may be challenging, as diagnostic tests are limited and may prove equivocal. This study aimed to investigate transcriptional differences between CETL and IMD, as a basis for further research on discriminating diagnostic biomarkers. We performed 100bp single-end sequencing on RNA extracted from formalin-fixed and paraffin-embedded skin biopsies from dogs with CETL and IMD, respectively. DESeq2 was used for principal component analysis (PCA) and differential gene expression analysis. Genes with significantly different expression were analyzed for enriched pathways using two different tools. The expression of selected genes and their proteins was validated by RT-qPCR and immunohistochemistry. PCA demonstrated the distinct gene expression profiles of CETL and IMD. In total, 503 genes were upregulated, while 4986 were downregulated in CETL compared to IMD. RT-qPCR confirmed the sequencing results for 5/6 selected genes tested, while the protein expression detected by immunohistochemistry was not entirely consistent. Our study revealed transcriptional differences between canine CETL and IMD, with similarities to human cutaneous lymphoma. Differentially expressed genes are potential discriminatory markers, but require further validation on larger sample collections.
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