Phytochemical screening and anti-oxidant activity of Sargassum wightii enhances the anti-bacterial activity against Pseudomonas aeruginosa

In this study, the phytochemical, phenolic, flavonoid and bioactive compounds were successfully screened from crude extract of Sargassum wightii by LC-MS analysis after NIST interpretation. Bacterial growth inhibition study result was shown with 24 mm zone inhibition at 200 mu g/mL concentration against P. aeruginosa. The increased phenolic content was much closed to gallic acid and the range was observed at 250 mu g/mL concentration. In addition, flavonoid contents of the algae extract was indicated more significant with rutin at 200 mu g/mL. In result, both the phenolic and flavonoid contents of the extract were more correlated with gallic acid and rutin. Further, the total anti-oxidant and DPPH radical scavenging activities were shown increased activity at 200 mu g/mL concentrations. Furthermore, the excellent anti-bacterial alteration result was observed at 200 mu g/mL concentration by minimum inhibition concentration. Therefore, the result was revealed that the marine algae Sargassum wightii has excellent phytochemical and anti-oxidant activities, and it has improved anti-bacterial activity against P. aeruginosa. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Automated summary

The study successfully screened phytochemicals, phenolic compounds, flavonoids, and bioactive compounds from crude extract of Sargassum wightii using LC-MS analysis. The extract showed excellent antioxidant and antibacterial activities, especially against P. aeruginosa, at 200 μg/mL concentration.