Journal
MICROMACHINES
Volume 12, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/mi12010030
Keywords
electrokinetic deterministic lateral displacement; charge-based separation; dielectrophoresis
Categories
Funding
- European Union [607350, 801367, 634890]
- Swedish Research council [2016-05739]
- NanoLund
- Swedish Research Council [2016-05739] Funding Source: Swedish Research Council
- H2020 Societal Challenges Programme [634890] Funding Source: H2020 Societal Challenges Programme
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By combining deterministic lateral displacement (DLD) with electrokinetics, this study demonstrates the sorting of cells based on differences in membrane and/or internal structures, and successfully separates different cell types in experiments. The significant change in zeta potential or dielectrophoretic mobility at different frequencies is utilized to achieve cell separation.
We show that by combining deterministic lateral displacement (DLD) with electrokinetics, it is possible to sort cells based on differences in their membrane and/or internal structures. Using heat to deactivate cells, which change their viability and structure, we then demonstrate sorting of a mixture of viable and non-viable cells for two different cell types. For Escherichia coli, the size change due to deactivation is insufficient to allow size-based DLD separation. Our method instead leverages the considerable change in zeta potential to achieve separation at low frequency. Conversely, for Saccharomyces cerevisiae (Baker's yeast) the heat treatment does not result in any significant change of zeta potential. Instead, we perform the sorting at higher frequency and utilize what we believe is a change in dielectrophoretic mobility for the separation. We expect our work to form a basis for the development of simple, low-cost, continuous label-free methods that can separate cells and bioparticles based on their intrinsic properties.
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