Leaf Venation and Morphology Help Explain Physiological Variation in Yucca brevifolia and Hesperoyucca whipplei Across Microhabitats in the Mojave Desert, CA

Different microclimates can have significant impact on the physiology of succulents that inhabit arid environments such as the Mojave Desert (California). We investigated variation in leaf physiology, morphology and anatomy of two dominant Mojave Desert monocots, Yucca brevifolia (Joshua tree) and Hesperoyucca whipplei, growing along a soil water availability gradient. Stomatal conductance (g(s)) and leaf thickness were recorded in the field at three different sites (north-western slope, south-eastern slope, and alluvial fan) in March of 2019. We sampled leaves from three individuals per site per species and measured in the lab relative water content at the time of g(s) measurements, saturated water content, cuticular conductance, leaf morphological traits (leaf area and length, leaf mass per area, % loss of thickness in the field and in dried leaves), and leaf venation. We found species varied in their g(s): while Y. brevifolia showed significantly higher g(s) in the alluvial fan than in the slopes, H. whipplei was highest in the south-eastern slope. The differences in g(s) did not relate to differences in leaf water content, but rather to variation in number of veins per mm(2) in H. whipplei and leaf width in Y. brevifolia. Our results indicate that H. whipplei displays a higher water conservation strategy than Y. brevifolia. We discuss these differences and trends with water availability in relation to species' plasticity in morphology and anatomy and the ecological consequences of differences in 3-dimensional venation architecture in these two species.

Automated summary

This study investigated the variation in leaf physiology, morphology, and anatomy of Yucca brevifolia and Hesperoyucca whipplei in different microclimates of the Mojave Desert. It found differences in stomatal conductance and leaf thickness, with H. whipplei displaying a higher water conservation strategy than Y. brevifolia. These differences were related to variations in leaf venation and leaf width.