Journal
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
Volume 10, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2020.610985
Keywords
Plasmodium falciparum; antimalarial drugs; artemisinin resistance; microarrays; Southern China
Categories
Funding
- Natural Science Foundation of Shanghai [18ZR1443400]
- WHO Mekong Malaria Programme [WP/08/MVP/000512, WP/10/MVP/005837]
- Howard Hughes Medical Institute
- Shanghai government
- National Institute of Allergy and Infectious Diseases at the National Institutes of Health [R01AI101713]
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The study identified artemisinin resistance in parasites from Southern China and demonstrated the utility of SNP microarrays for large-scale parasite molecular epidemiology. The prevalence of resistance haplotypes and specific genetic loci associated with delayed parasite clearance were analyzed, revealing a substructure within parasite populations in Yunnan province and Hainan island.
Emerging artemisinin resistance in Southeast Asia poses a significant risk to malaria control and eradication goals, including China's plan to eliminate malaria nationwide by 2020. Plasmodium falciparum was endemic in China, especially in Southern China. Parasites from this region have shown decreased susceptibility to artemisinin and delayed parasite clearance after artemisinin treatment. Understanding the genetic basis of artemisinin resistance and identifying specific genetic loci associated with this phenotype is crucial for surveillance and containment of resistance. In this study, parasites were collected from clinical patients from Yunnan province and Hainan island. The parasites were genotyped using a P. falciparum-specific single nucleotide polymorphism (SNP) microarray. The SNP profiles examined included a total of 27 validated and candidate molecular markers of drug resistance. The structure of the parasite population was evaluated by principal component analysis by using the EIGENSOFT program, and ADMIXTURE was used to calculate maximum likelihood estimates for the substructure analysis. Parasites showed a high prevalence of resistance haplotypes of pfdhfr and pfdhps and moderate prevalence of pfcrt. There was no mutation identified on pfmdr1. Candidate SNPs on chromosomes 10, 13, and 14 that were associated with delayed parasite clearance showed a low prevalence of mutants. Parasites from Southern China were clustered and separated from those from Southeast Asia. Parasites from Yunnan province were substructured from parasites from Hainan island. This study provides evidence for a genomic population with drug resistance in Southern China and also illustrates the utility of SNP microarrays for large-scale parasite molecular epidemiology.
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