Journal
ELIFE
Volume 9, Issue -, Pages -Publisher
ELIFE SCIENCES PUBLICATIONS LTD
DOI: 10.7554/eLife.56205
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Funding
- Austrian Science Fund [P30623, I2514, W1224]
- Herzfelder'sche Familienstiftung [P30623]
- Hochschuljubilaumsstiftung der Stadt Wien [H-327123/2018]
- National Institute of General Medical Sciences [P20GM103423, P20GM104318]
- Universite Libre de Bruxelles (ULB)
- Mount Desert Island Biological Laboratory
- Belgian National Fund for Scientific Research
- Region Wallonne (DGO6) [1810070]
- Austrian Science Fund (FWF) [P30623] Funding Source: Austrian Science Fund (FWF)
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Our knowledge about the repertoire of ribosomal RNA modifications and the enzymes responsible for installing them is constantly expanding. Previously, we reported that NSUN-5 is responsible for depositing m(5)C at position C2381 on the 26S rRNA in Caenorhabditis elegans. Here, we show that NSUN-1 is writing the second known 26S rRNA m(5)C at position C2982. Depletion of nsun-1 or nsun-5 improved thermotolerance and slightly increased locomotion at midlife, however, only soma-specific knockdown of nsun-1 extended lifespan. Moreover, somaspecific knockdown of nsun-1 reduced body size and impaired fecundity, suggesting non-cell-autonomous effects. While ribosome biogenesis and global protein synthesis were unaffected by nsun-1 depletion, translation of specific mRNAs was remodeled leading to reduced production of collagens, loss of structural integrity of the cuticle, and impaired barrier function. We conclude that loss of a single enzyme required for rRNA methylation has profound and highly specific effects on organismal development and physiology.
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