Osteopontin-induced lncRNA HOTAIR expression is involved in osteoarthritis by regulating cell proliferation

BackgroundOsteopontin plays critical roles in osteoarthritis (OA) by regulating the functions of osteoclasts. It is known that osteopontin can induce the expression of lncRNA HOX transcript antisense RNA (HOTAIR), indicating the involvement of HOTAIR in OA. This study was carried out to investigate the role of HOTAIR in OA.MethodsSynovial fluid was extracted from both OA patients (n=58) and healthy controls (n=58). Expression of osteopontin and HOTAIR in synovial fluid was determined by RT-qPCR. Osteopontin was used to treat chondrocytes at dosages of 0, 1, 5 and 10 mu g/ml, followed by measurement of HOTAIR expression by RT-qPCR. The role of osteopontin and HOTAIR overexpression, as well as HOTAIR knockdown in regulating the proliferation of chondrocytes was analyzed by cck-8 assay.ResultsHOTAIR was upregulated in OA. A positive correlation between HOTAIR and osteopontin was observed. In the primary chondrocytes, osteopontin treatment increased HOTAIR expression, while HOTAIR overexpression and knockdown failed to significantly affect osteopontin expression. In addition, osteopontin and HOTAIR overexpression increased chondrocyte proliferation, while HOTAIRE knockdown decreased chondrocyte proliferation. In addition, HOTAIR knockdown reduced the effects of osteopontin treatment on cell proliferation.ConclusionsOsteopontin-induced HOTAIR expression is involved in osteoarthritis by regulating cell proliferation.

Automated summary

Osteopontin-induced HOTAIR expression is involved in osteoarthritis by regulating cell proliferation. The study found that HOTAIR was upregulated in OA and showed a positive correlation with osteopontin. While osteopontin treatment increased HOTAIR expression in primary chondrocytes, HOTAIR overexpression and knockdown did not significantly affect osteopontin expression. Additionally, both osteopontin and HOTAIR overexpression increased chondrocyte proliferation, while HOTAIR knockdown decreased chondrocyte proliferation and reduced the effects of osteopontin treatment on cell proliferation.