Structure Analysis and Antioxidant Activity of a Novel Polysaccharide from Katan Seeds

In the present work, a novel water-soluble polysaccharide (LWSP) was purified from Katan seeds. Polysaccharide was structurally characterized by NMR spectroscopic analysis, thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) analysis, X-ray diffraction (XRD), and UV absorption. TLC and HPLC showed that LWSP was a polysaccharide consisted mainly of glucose, mannose, xylose, and arabinose. The FTIR spectrum and UV absorption proved polysaccharide characteristic of LWSP. According to XRD, LWSP presented a semicrystalline behavior. The molecular weight was estimated as 64.56 kDa. Results obtained through C-13 and H-1 nuclear magnetic resonance (NMR) indicated that LWSP is consisted of four monosaccharide residues with alpha and beta anomers. Physicochemical and antioxidant properties of LWSP were also investigated. Results revealed that LWSP exhibited interesting 1,1-diphenyl-2-picrylhydrazyl (DPPH) (IC50=4.48 mg/ml) and chelating activity (IC50=4.79 mg/ml), and it displayed moderate reductive capacities. Overall, the findings suggested that LWSP is a promising source of natural additives in various industries fields.

Automated summary

In this study, a novel water-soluble polysaccharide (LWSP) was extracted from Katan seeds and characterized through various analytical techniques. The LWSP was found to mainly consist of glucose, mannose, xylose, and arabinose, with a semicrystalline behavior and a molecular weight of 64.56 kDa, showing promising antioxidant properties.