Journal
PLOS BIOLOGY
Volume 18, Issue 11, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pbio.3000965
Keywords
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Categories
Funding
- CIHR [MOP-123514, FS-154310, FDN143238]
- NSERC [RGPIN 288338-2010, 2018-04364, RGPIN-2017-05005]
- Brain Canada
- NIH [U01 NS094246, UO1 NS090565, 2R01-DA029639, 1R01-MH12297101, 1RF1-NS113287]
- U.S.-Israel Binational Science Foundation [2014509]
- NSF [1848029, 1734870]
- Foundation of Westlake University
- Conrad F. Harrington Fellowship from the faculty of medicine at the University of McGill
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1848029] Funding Source: National Science Foundation
- Division Of Undergraduate Education
- Direct For Education and Human Resources [1734870] Funding Source: National Science Foundation
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Near-infrared (NIR) genetically encoded calcium ion (Ca2+) indicators (GECIs) can provide advantages over visible wavelength fluorescent GECIs in terms of reduced phototoxicity, minimal spectral cross talk with visible light excitable optogenetic tools and fluorescent probes, and decreased scattering and absorption in mammalian tissues. Our previously reported NIR GECI, NIR-GECO1, has these advantages but also has several disadvantages including lower brightness and limited fluorescence response compared to state-of-the-art visible wavelength GECIs, when used for imaging of neuronal activity. Here, we report 2 improved NIR GECI variants, designated NIR-GECO2 and NIR-GECO2G, derived from NIR-GECO1. We characterized the performance of the new NIR GECIs in cultured cells, acute mouse brain slices, and Caenorhabditis elegans and Xenopus laevis in vivo. Our results demonstrate that NIR-GECO2 and NIR-GECO2G provide substantial improvements over NIR-GECO1 for imaging of neuronal Ca2+ dynamics.
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