Journal
ADVANCED HEALTHCARE MATERIALS
Volume 10, Issue 7, Pages -Publisher
WILEY
DOI: 10.1002/adhm.202001755
Keywords
colorimetric; LAMP; MRSA; paper‐ based analytical devices; silver nanoplates
Funding
- State Secretariat for Education, Research and Innovation (SERI) [2016.0728]
- ETH Zurich
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This study presents a rapid, highly sensitive, and quantitative colorimetric paper-based analytical device (PAD) based on silver nanoplates and loop-mediated isothermal amplification (LAMP), demonstrating its application in single copy detection. The PAD allows qualitative analysis by the naked eye and quantitative analysis using a smartphone camera, running the entire assay in situ on the paper surface.
A rapid, highly sensitive, and quantitative colorimetric paper-based analytical device (PAD) based on silver nanoplates (AgNPls) and loop-mediated isothermal amplification (LAMP) is presented. It is shown that cauliflower-like concatemer LAMP products can mediate crystal etching of AgNPls, with a threefold signal enhancement versus linear dsDNA. Methicillin-resistant Staphylococcus aureus (MRSA), an antimicrobial resistant bacterium that poses a formidable risk with persistently high mortality, is used as a model pathogen. Due to the excellent color contrast provided by AgNPls, the PAD allows qualitative analysis by the naked eye and quantitative analysis using a smartphone camera, with detection limits down to a single copy in just 30 min, and a linear response from 1 to 10(4) copies (R-2 = 0.994). The entire assay runs in situ on the paper surface, which drastically simplifies operation of the device. This is the first demonstration of single copy detection using a colorimetric readout, and the developed PAD shows great promise for translation into an ultrasensitive gene-based point-of-care test for any infectious disease target, via modification of the LAMP primer set.
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