Journal
NATURE COMMUNICATIONS
Volume 12, Issue 1, Pages -Publisher
NATURE RESEARCH
DOI: 10.1038/s41467-020-20686-z
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Funding
- Engineering and Physical Sciences Research Council of the UK [EP/N008235/1]
- Biotechnology and Biological Sciences Research Council [BB/P026508/1, BB/T007176/1]
- Royal Society University Research Fellowship [UF160152]
- European Research Council (ERC) under the Horizon 2020 Research and Innovation Programme (ERC-STG) [851667-NANOCELL]
- EPSRC DTP studentship
- EPSRC [EP/P020259/1]
- STFC
- BBSRC [BB/T007176/1, BB/P026508/1] Funding Source: UKRI
- EPSRC [EP/N008235/1] Funding Source: UKRI
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DNA-PAINT suffers from high background signals and non-specific binding, but Repeat DNA-PAINT can suppress background noise, prevent photoinduced site loss, and decrease sampling time.
DNA-PAINT is a versatile optical super-resolution technique relying on the transient binding of fluorescent DNA 'imagers' to target epitopes. Its performance in biological samples is often constrained by strong background signals and non-specific binding events, both exacerbated by high imager concentrations. Here we describe Repeat DNA-PAINT, a method that enables a substantial reduction in imager concentration, thus suppressing spurious signals. Additionally, Repeat DNA-PAINT reduces photoinduced target-site loss and can accelerate sampling, all without affecting spatial resolution. DNA-PAINT is a super-resolution imaging technique which suffers from high background signals and non-specific binding. Here the authors report Repeat DNA-PAINT which is capable of supressing background noise and preventing photoinduced site loss, as well as decreasing the time taken for the sampling process.
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