Long non-coding RNA H19 regulates Bcl-2, Bax and phospholipid hydroperoxide glutathione peroxidase expression in spontaneous abortion

Spontaneous abortion (SA) is the most frequently occurring pregnancy disorder and is a serious threat to women's health. Identifying novel risk factors and the molecular mechanisms underlying SA are important. The present study reported that the RNA expression levels of long non-coding RNA H19 were lower in SA group compared with those in the control group, and the expression of Bax was increased and levels of Bcl-2 and phospholipid hydroperoxide glutathione peroxidase (GPX4) were decreased in SA group at both the mRNA and protein levels. H19 expression was positively correlated with Bcl-2 and GPX4 expression and negatively linked with Bax levels. It was demonstrated that silencing H19 downregulated Bcl-2 and GPX4 expression and upregulated Bax expression at both the mRNA and protein levels in HTR-8/SVneo trophoblast cells. In conclusion, the present findings suggested that H19 has important roles in SA by promoting apoptosis and ferroptosis.

Automated summary

This study revealed the associations between the expression levels of long non-coding RNA H19, Bax, Bcl-2, and GPX4 in spontaneous abortion (SA), showing their importance in regulating apoptosis and ferroptosis processes in SA.