Journal
VIRUSES-BASEL
Volume 13, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/v13020171
Keywords
HIV-1; Uganda; recombinant; Gag-Pol; protein domains
Categories
Funding
- IAVI
- Bill and Melinda Gates Foundation
- Ministry of Foreign Affairs of Denmark
- Irish Aid
- Ministry of Finance of Japan
- Ministry of Foreign Affairs of the Netherlands
- Norwegian Agency for Development Cooperation (NORAD)
- United Kingdom Department for International Development (DFID)
- United States Agency for International Development (USAID)
- Yerkes National primate research Centre base grant through the office of Research infrastructure programs [OD P51OD11132]
- MRC [MC_EX_MR/L016273/1, MC_UU_00027/1] Funding Source: UKRI
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This study focused on Ugandan viruses from newly infected patients and investigated the role of Gag-Pol genes in replication capacity of HIV-1. The research found diversity in functional protein domains across the Gag-Pol region, with differences in the Gag-p6 domain frequently associated with higher in vitro replication levels.
The ability to efficiently establish a new infection is a critical property for human immunodeficiency virus type 1 (HIV-1). Although the envelope protein of the virus plays an essential role in receptor binding and internalization of the infecting virus, the structural proteins, the polymerase and the assembly of new virions may also play a role in establishing and spreading viral infection in a new host. We examined Ugandan viruses from newly infected patients and focused on the contribution of the Gag-Pol genes to replication capacity. A panel of Gag-Pol sequences generated using single genome amplification from incident HIV-1 infections were cloned into a common HIV-1 NL4.3 pol/env backbone and the influence of Gag-Pol changes on replication capacity was monitored. Using a novel protein domain approach, we then documented diversity in the functional protein domains across the Gag-Pol region and identified differences in the Gag-p6 domain that were frequently associated with higher in vitro replication.
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